Cellulose production from<Emphasis Type="Italic">Gluconobacter oxydans</Emphasis> TQ-B2 |
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Authors: | Shiru?Jia Hongyu?Ou Guibing?Chen Email author" target="_blank">DuBok?ChoiEmail author KiAn?Cho Mitsuyasu?Okabe Wol?Suk?Cha |
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Institution: | (1) Department of Biochemical Engineering, Tianjin University of Science and Technology, 300222 Tianjin, China;(2) Department of Environmental Engineering, Cho-dang University, 419, Songnam-ri, Muan-up, Muan-kun, 534-800 Chonnam, Korea;(3) Biochemical Engineering Lab, Applied Biological Chemistry, Shizuoka University, 836 Ohya, 422 Shizuoka City, Japan;(4) Department of Chemical Engineering, Chosun University, Seosuk dong 375, 501-759 Kwangju, Korea |
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Abstract: | Gluconobacter oxydans that produces the cellulose was isolated. In order to confirm the chemical features of cellulose, various spectrophtometeric
analysis were carried out using electron microscopy, X-ray diffractogram, and CP/MAS13C NMR. The purified cellulose was found to be identical to that ofAcetobacter xylinum. For effective production of cellulose, the various carbon and nitrogen sources, mixture of calcium and magnesium ions, and
biotin concentration were investigated in flask cultures. Among the various carbon sources, glucose and sucrose were found
to be best for the production of cellulose, with maximum concentration of 2.41 g/L obtained when a mixture of 10 g/L of each
glucose and sucrose were used. With regard to the nitrogen sources, when 20 g/L of yeast extract was used, the maximum concentration
of bacterial cellulose was reached. The concentration of cellulose was increased with mixture of 2 mM of each Ca2+ and Mg2+. The optimum biotin concentration for the production of cellulose was in the range of 15 to 20 mg/L. At higher biotin concentration
(25–35 mg/L), the bacterial cellulose production was lower. |
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Keywords: | cellulose Gluconobacter oxydans red tea fungi |
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