Stable expression of the GUS reporter gene in chrysanthemum depends on binary plasmid T-DNA |
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Authors: | Jan de Jong Marleen M J Mertens Wim Rademaker |
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Institution: | (1) DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700 AA Wageningen, The Netherlands;(2) Research Station for Ornamental Plant Growing, Caritasstraat 21, B-9990 Mollo, Belgium |
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Abstract: | Three chrysanthemum (Dendranthema grandiflora) cultivars were cocultivated with 2 Agrobacterium tumefaciens strains in combination with 4 pBIN19 derived binary plasmids, all carrying the Nosnptll selection gene and 35Sgus(intron) reporter gene. All binary plasmids transferred DNA to chrysanthemum explants but only pMOG410 gave good stable expression of GUS. This plasmid differs from the other plasmids in 2 aspects: 1) It carries a restored nptll gene and 2) the selection gene is positioned at the left border side of the reporter gene. Cocultivation with AGLO(pMOG410) yielded up to 13 GUS positive shoots per 100 explants. The presence of the gus and nptll gene in recovered shoots was confirmed by PCR and Southern blot analysis. |
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Keywords: | Agrobacterium transformation T-DNA |
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