Separation and Characterization of Four Forms of β-N-Acetylhexosaminidase from Chicken Brain |
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| Authors: | José A Rodríguez-Hernández Josefa Martín-Barrientos Carmen Sánchez-Bernal José A Cabezas |
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| Institution: | Department of Biochemistry, Faculty of Biology, University of Salamanca;and C.S.I.C., Salamanca, Spain |
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| Abstract: | Chicken brain beta-N-acetylhexosaminidases from embryos (16 and 21 days old), newborns (1 and 4 days old), and adults (3 1/2 months and 2 years old) were separated into four different forms by ion exchange chromatography on diethylaminoethyl-cellulose. Three of these forms were "acid" hexosaminidases (I, IIA, and IIB), and the fourth was a "neutral" form. Throughout development of the chicken, forms IIA and III maintained the same activity ratio, whereas that for form I decreased and that for form IIB showed an increase. |
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| Keywords: | Chicken brain β-N-Acetylhexosaminidase Ion exchange chromatography Molecular forms |
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