Protease digestion of hepatitis A virus: disparate effects on capsid proteins, antigenicity, and infectivity. |
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Authors: | S M Lemon E Amphlett D Sangar |
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Affiliation: | Department of Medicine, University of North Carolina, Chapel Hill 27599-7030. |
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Abstract: | High concentrations of either trypsin or chymotrypsin caused nearly complete cleavage of capsid protein VP2 of hepatitis A virus but did not significantly reduce the infectivity, thermostability, or antigenicity of the virus. Chymotrypsin also had a lesser effect on VP1. These findings indicate the presence of a protease-accessible VP2 surface site which neither contributes significantly to the dominant antigenic site nor plays a role in the attachment of the virus to putative cell receptors. |
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