An Improved Method for the Purification of "Light" Noradrenaline Vesicles from Rat Vas Deferens: Some Biochemical Characteristics |
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Authors: | M. F. Willems W. P. De Potter |
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Affiliation: | Universitaire Instelling Antwerpen, Department of Medicine, Wilrijk, Belgium |
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Abstract: | "Light" noradrenaline storage vesicles from nerve endings have been isolated by differential centrifugation and differential gradient centrifugation. They have been further purified by isopycnic sucrose/D2O centrifugation. By using these centrifugation techniques, we obtained an isopycnic gradient fraction in which noradrenaline was enriched about 41 times versus a total homogenate. This factor could be raised to 61 by using seminal ducts of castrated rats. Comparison of the distribution patterns in sucrose/D2O isopycnic gradients indicated that light noradrenaline vesicles of nerve endings contain Mg2+-stimulated ATPase and ATP, but that only a minor part of the dopamine beta-hydroxylase can be associated with these vesicles. |
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Keywords: | ATP Dopamine β-hydrox-ylase Mg2+ ATPase Noradrenaline Vesicles Vas deferens |
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