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Development and validation of a mismatch amplification mutation PCR assay to monitor the dissemination of an emerging variant of Vibrio cholerae O1 biotype El Tor |
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| Authors: | Morita Masatomo Ohnishi Makoto Arakawa Eiji Bhuiyan N A Nusrin Suraia Alam Munirul Siddique A K Qadri Firdausi Izumiya Hidemasa Nair G Balakrish Watanabe Haruo |
| Affiliation: | National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo, 162-8640, Japan. |
| Abstract: | A mismatch amplification mutation PCR assay was developed and validated for rapid detection of the biotype specific cholera toxin B subunit of V. cholerae O1. This assay will enable easy monitoring of the spread of a new emerging variant of the El Tor biotype of V. cholerae O1. |
| Keywords: | altered El Tor strain biotype cholera toxin Vibrio cholerae O1 |
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