Developmental changes in membrane-bound enzymes of Dictyostelium discoideum detected by concanavalin A-Sepharose affinity chromatography.

Plasma membrane-enriched fractions isolated from $\text{Dictyostelium discoideum}$ at early stages of development were detergent extracted and subjected to affinity chromatography on a concanavalin A-Sepharose column. Alkaline phosphatase, 5′-nucleotidase, and cAMP phosphodiesterase activities were totally bound to the column when logarithmically growing cells were examined. As the cells entered development, however, a progressive decrease in the ability of these activities to bind to the affinity column was evident.