Evaluation of a real-time PCR kit for detecting Escherichia coli O157 in bovine fecal samples |
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| Authors: | Bono James L Keen James E Miller Laura C Fox James M Chitko-McKown Carol G Heaton Michael P Laegreid William W |
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| Institution: | U.S. Meat Animal Research Center, Agricultural Research Service, U.S. Department of Agriculture, Clay Center, Nebraska 68933-0166, USA. bono@email.marc.usda.gov |
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| Abstract: | A commercially available real-time, rapid PCR test was evaluated for its ability to detect Escherichia coli O157. Both the sensitivity and specificity of the assay were 99% for isolates in pure culture. The assay detected 1 CFU of E. coli O157:H7 g(-1) in artificially inoculated bovine feces following enrichment. |
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