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A new mutation protocol for obtaining auxotrophic mutants of the yeastPhaffia rhodozyma
Authors:Zs Palágyi  Á Nagy  Cs Vágvölgyi  L Ferenczy
Institution:(1) Department of Microbiology, Attila József University, P.O.Box 533, H-6701 Szeged, Hungary
Abstract:Summary A new mutation strategy, which involves gamma-irradiation of cells followed by a selective enzymatic enrichment step, was worked out to obtain auxotrophic mutants from the astaxanthin-producing yeast P. rhodozyma. Under the optimized conditions described, different mutants suitable for strain improvement were isolated.
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