| 非水介质大孔树脂分离纯化虾壳中虾青素 |
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| 引用本文： | 陈志强,金杨,任璐.非水介质大孔树脂分离纯化虾壳中虾青素[J].生物加工过程,2009,7(3):39-42. |
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| 作者姓名： | 陈志强 金杨 任璐 |
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| 作者单位： | 1. 天津科技大学,食品工程与生物技术学院,教育部食品营养与安全重点实验室,天津,300457;天津市尖峰天然产物研究开发有限公司,天津,300457
2. 天津科技大学,食品工程与生物技术学院,教育部食品营养与安全重点实验室,天津,300457 |
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| 基金项目： | 天津科技大学引进人才科研启动基金 |
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| 摘 要： | 通过7种大孔树脂对虾青素的静态吸附容量和解析率等指标的考察，筛选出AB-8大孔吸附树脂，用于分离虾壳中虾青素，同时利用高效液相色谱（HPLC）法测量虾青素的含量。结果表明，AB-8树脂对虾青素的吸附量为（24．17±0．5）mg／g，解吸率为95．2％，最大上样量（每g干树脂）以虾青素计为（23．07±0．2）mg，并确定用8倍量柱床体积的乙酸乙酯为洗脱剂，纯化所得虾青素的纯度为14．73％。
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| 关 键 词： | 大孔树脂 虾壳 虾青素 吸附 |
Separation and purification of astaxanthin from shrimp waste by non-aqueous medium macroporous resin |
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| CHEN Zhi-qiang,JIN Yang,REN Lu.Separation and purification of astaxanthin from shrimp waste by non-aqueous medium macroporous resin[J].Chinese Journal of Bioprocess Engineering,2009,7(3):39-42. |
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| Authors: | CHEN Zhi-qiang JIN Yang REN Lu |
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| Institution: | 1. College of Food Engineering and Biotechnology, Key Laboratory of Food Nutrition and Safety of Ministry of Education, Tianjin University of Science ＆ Technology, Tianjin 300457, China; 2. Jianfeng Natural Product R＆D Co. Ltd. , Tianjin 300457, China) |
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| Abstract: | Seven types of macroporous adsorption resins were screened with respect to the static adsorption capacity and desorption ratio of astaxantin of shrimp waste and the AB-8 type was chosen as the best one. Astaxantin of shrimp waste was analyzed by high performance liquid chromatography. The results showed that the static adsorption quantity reached （24. 17 ±0. 5） rag/g, the desorption rate was 95.2%. The maximal adsorption quantity of astaxantin was （23.07 ± 0. 2） mg/g dry resin, the determined elution solvent was eight-fold column volume of ethlacetate, and the product purity was 14. 73%. |
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| Keywords: | macroporous resin shrimp waste astaxantin adsorption |
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