Indigenous organophosphate-degrading (opd) plasmid pCMS1 of Brevundimonasdiminuta is self-transmissible and plays a key role in horizontal mobility of the opd gene |
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Authors: | Pandeeti Emmanuel Vijay Paul Chakka Deviprasanna Pandey Jay Prakash Siddavattam Dayananda |
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Affiliation: | a Dept. of Animal Sciences, School of Life Sciences, University of Hyderabad, Hyderabad 500 046, India |
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Abstract: | A fosmid library of the 66kb indigenous organophosphate-degrading (opd) plasmid pCMS1 of Brevundimonas diminuta was tagged with mini-transposon EZTn5 , to determine its sequence using transposon-specific primers. The sequence revealed the presence of a number of tra genes suggesting their role in conjugal transfer of pCMS1. Consistent with the presence of the tra genes, the B. diminuta plasmid, pCMS1::tet, generated by replacing the opd gene with opd::tet, served as a donor for transferring pCMS1::tet into recipient strain Pseudomonas putida. The self-transmissibility of the opd-containing plasmid pCMS1 and the existence of identical opd genes on otherwise dissimilar plasmids suggests a probable role of indigenous opd plasmids like pCMS1 in transferring the opd gene among soil bacteria. |
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Keywords: | Indigenous plasmids Horizontal mobility Conjugation Catabolic plasmids Phosphotriesterase |
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