Luminal responses to bradykinin on the isolated canine tracheal epithelium: effects of bradykinin antagonists期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

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Luminal responses to bradykinin on the isolated canine tracheal epithelium: effects of bradykinin antagonists

Authors:	P K Rangachari B Donoff R J Vavrek and J M Stewart

Institution:	^a Intestinal Disease Research Unit, Department of Medicine, McMaster University, Hamilton, Ontario, Canada ^b Department Biochemistry, University of Colorado, School of Medicine, Denver, CO, USA

Abstract:	We have tested the ability of several B₂ antagonists on the responses of the open-circuited isolated canine tracheal epithelium to the luminal addition of Bradykinin (BK), Lys-BK, and substance P (SP). All three peptides produced biphasic changes in transmural potential difference (PD), an initial decrease (dip) followed by an increase (rise). The B₂ antagonists -Arg^o Hyp³,Thi^5,8, -Phe⁷]BK (B5630) reversibly inhibited both the dips and the rise with IC₅₀ values of 2.01 · 10^?8 and 1.54 · 10^?7 M, respectively. The responses to SP were unaffected even with high concentrations of the antagonist. Other antagonists tested -Phe^1,7,Thi^5,8]BK (B4158), -Phe^2,7]BK (B4404), and -Phe⁷,Hyp⁸]BK (B5092) were ineffective.

Keywords:	Airway epithelium Bradykinin antagonists Peptide receptors Anion channels
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