A Simple Method for Searching Intracellular Space Structures in Plant Tissues

After being fixed, plant leaves were embedded in polyethylene glycol (PEG, molecular weight 4000) and sectioned 5–10μm in thickness. PEG in the slices was dissolved during the routine process and the slices were observed under Hitachi S-450 scanning electron microscope By using this method, it is easy to study the space structures of organelles such as chloroplasts, mitochondria, nucleus, nucleolus, etc. as well as their space inter relations.