Studies on Stem Tissue Culture and Organgenesis of Aloe vera

tem segments of Aloe vera were cultured on MS medium with different hormones and successfully regenerated into a large number of plantlets via callus-globoid-adventitious bud. In application of various cytokinins, the effect of zeatin on organgenesis was better than kinetm and the enhanced effect was apparent when NAA was combined with zeatin or kinetin on MS medium. The best results were obtained on the medium with zeatin 2ppm+NAA 0.5ppm. With scanning electron microscope and histocytological method the observation showed that the globoid which is a cell mass with hollow on the top originated from the surface of callus. After differentiation of scale primordium twice, the primordium of adventitious bud was formed between scale primordia. Finally, the adventitious bud was developed into plantlet. Therefore the globid can be considered as a proliferation unit producing adventitious bud.