Prevalence of 1-aminocyclopropane-1-carboxylate deaminase in Rhizobium spp |
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| Authors: | Ma Wenbo Sebestianova Stepanka B Sebestian Jiri Burd Genrich I Guinel Frédérique C Glick Bernard R |
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| Institution: | (1) Department of Biology, University of Waterloo, Waterloo, ON, N2L 3G1, Canada;(2) Department of Genetics, University of South Bohemia, Ceske Budejovice, Czech Republic;(3) Laboratory of Biomembranes, University of South Bohemia, Ceske Budejovice, Czech Republic;(4) Department of Biology, Wilfrid Laurier University, Waterloo, ON, N2L 3C5, Canada |
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| Abstract: | This is the first report documenting the presence of 1-aminocyclopropane-1-carboxylate (ACC) deaminase in Rhizobium. This enzyme, previously found in free-living bacteria, yeast and fungi, degrades ACC, the immediate precursor of ethylene in higher plants. Thirteen different rhizobial strains were examined by Southern hybridization, Western blots and ACC deaminase enzyme assay. Five of them tested positive for ACC deaminase. Induction of the expression of ACC deaminase was examined in one of the positively tested strains, Rhizobium leguminosarum bv. viciae 128C53K. This rhizobial ACC deaminase had a trace basal level of expression without ACC, but could be induced by a concentration of ACC as low as 1 microM. The more ACC added to this Rhizobium the higher the expression level of the ACC deaminase. |
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| Keywords: | ACC deaminase Ethylene Nodulation Rhizobium |
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