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Carbonic Anhydrase of Chlamydomonas reinhardtii. Cellular Distribution and Subunit Constitution in Two Strains
Authors:Yagawa  Yuriko; Muto  Shoshi; Miyachi  Shigetoh
Institution:Institute of Applied Microbiology, University of Tokyo Bunkyo-ku, Tokyo 113, Japan
Abstract:Cellular distribution and subunit constitution of carbonic anhydrase(CA) were compared between the CBSC strain of Chlamydomonasreinhardtii (purchased from Carolina Biological Suuply Co.)and C. reinhardtii C-9. CAs existed exclusively outside theplasma membrane inboth strains. Mol wts of the monomer and theholoenzyme from both strains were 35 kDa and 115–117 kDa,respectively. These results show that the CAs of both strainsare identical or verysimilar. 1Present address: National Institute for Basic Biology, Myodaiji,Okazaki 444, Japan. (Received June 29, 1987; Accepted October 9, 1987)
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