| 蒜油对MRSA菌株的抗菌作用及作用机制研究 |
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| 引用本文： | 黄红莹,娄强,李小红,姬新颖,朱江木,张虎,马远方.蒜油对MRSA菌株的抗菌作用及作用机制研究[J].中国微生态学杂志,2011,23(5):429-431. |
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| 作者姓名： | 黄红莹 娄强 李小红 姬新颖 朱江木 张虎 马远方 |
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| 作者单位： | 河南大学,医学院免疫学研究所;细胞与分子免疫重点实验室,河南,开封,475004 |
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| 摘 要： | 目的研究蒜油对MRSA菌株的抗菌作用及其作用机制,为治疗MRSA菌株感染提供实验依据。方法纸片法药敏试验测抑菌环直径,微量稀释法测其最低抑菌浓度(MIC),酶标仪测定不同时间MRSA菌株生长A640值,革兰染色镜检观察蒜油对MRSA菌株细胞壁的影响,SDS-PAGE凝胶电泳分析蒜油对MRSA菌株蛋白的影响。结果蒜油对MRSA菌株具有明显抗菌作用,其MIC和MBC分别为10mg/mL和20 mg/mL;1/2MIC和1MIC的蒜油作用MRSA菌株后,菌株生长曲线平缓,无对数期;且其对对数期细菌有明显杀菌作用;蒜油对MRSA菌株细胞壁无明显影响,凝胶电泳分析有特征条带出现。结论蒜油对MRSA菌株具有明显抗菌作用,其杀菌机制与蛋白合成有关。
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| 关 键 词： | MRSA菌株 蒜油 抗菌作用 |
The study of antibacterial effect and related mechanism of garlic oil on MRSA strain |
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| HUANG Hong-ying,LOU Qiang,LI Xiao-hong,JI Xin-ying,ZHU Jiang-mu,ZHANG Hu,MA Yuan-fang.The study of antibacterial effect and related mechanism of garlic oil on MRSA strain[J].Chinese Journal of Microecology,2011,23(5):429-431. |
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| Authors: | HUANG Hong-ying LOU Qiang LI Xiao-hong JI Xin-ying ZHU Jiang-mu ZHANG Hu MA Yuan-fang |
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| Institution: | HUANG Hong-ying,LOU Qiang,LI Xiao-hong,JI Xin-ying,ZHU Jiang-mu,ZHANG Hu,MA Yuan-fang(Key Laboratory of Cellular and Molecular Immunology,Institute of Immunology,Medical School of Henan University,Kaifeng 475004,China) |
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| Abstract: | Objective To investigate the antibacterial effect and related mechanism of garlic oil on MRSA strain,provide experimental evidence for treating MRSA infection.Method The inhibition zone diameter was examined by paper disk method;the MIC was determined by dilution method;the growth curve of the MRSA strain was assessed by Thermo Microplate Reader;the effect of garlic oil on the cell wall components was observed under microscopy associated with gram strain;and the whole-cell protein profile of MRSA strain was... |
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| Keywords: | MRSA strain Garlic oil Antibacterial effect |
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