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奶牛阴道菌群分析与乳酸菌的分离及鉴定
引用本文:侯荣伦,程超,付艳茹,刘彦民,李利平,郝永清,黄少磊.奶牛阴道菌群分析与乳酸菌的分离及鉴定[J].中国微生态学杂志,2011,23(5):393-397.
作者姓名:侯荣伦  程超  付艳茹  刘彦民  李利平  郝永清  黄少磊
作者单位:1. 内蒙古农业大学兽医学院,内蒙古,呼和浩特,010018
2. 内蒙古双奇药业股份有限公司,内蒙古,呼和浩特,010010
3. 呼和浩特职业学院,内蒙古,呼和浩特,010051
4. 内蒙古农牧业局兽药监察所,内蒙古,呼和浩特,010010
基金项目:呼和浩特市科技攻关项目
摘    要:目的研究奶牛阴道菌群,并从健康奶牛阴道分离出产酸能力很强的乳酸菌。方法采用常规的方法对奶牛阴道进行细菌的分离及鉴定,并进行菌群分析。结果健康奶牛阴道优势菌群主要为乳酸菌(P<0.01),屡配不孕奶牛阴道优势菌群主要为金黄色葡萄球菌(P<0.01);从健康奶牛阴道分离出的乳酸菌为55株,其中产酸能力很强的6株乳酸菌鉴定结果分别为Lactobacillus plantarum、Lactobacillus brevis、Enterococcus faecalis、Lactococcus garvieae、Lactobacillus kitasatonis和Lactobacillus amylovorus。结论奶牛阴道菌群中分离的6株乳酸菌可作为潜在的奶牛阴道微生态制剂进行深入研究。

关 键 词:奶牛阴道  菌群  乳酸菌  分离与鉴定

Analysis of microbial flora in dairy cow's vagina, and the isolation and identification of lactobacillus
HOU Rong-lun,CHEN Chao,FU Yan-ru,LIU Yan-min,LI Li-ping,HAO Yong-qing,HUANG Shao-lei.Analysis of microbial flora in dairy cow's vagina, and the isolation and identification of lactobacillus[J].Chinese Journal of Microecology,2011,23(5):393-397.
Authors:HOU Rong-lun  CHEN Chao  FU Yan-ru  LIU Yan-min  LI Li-ping  HAO Yong-qing  HUANG Shao-lei
Institution:HOU Rong-lun1,CHEN Chao2,FU Yan-ru3,LIU Yan-min2,LI Li-ping4,HAO Yong-qing1,HUANG Shao-lei2(1.College of Veterinary Medicine of Inner Mongolia Agricultural University,Huhhot 010018,China,2.Inner Mongolia ShuangQi Pharmaceutical Co.Ltd.,Huhhot 010010,3.Hohhot Vocational College,Huhhot 010051,4.Inner Mongolia Institute of Veterinary Drugs Control,China)
Abstract:Objective To Analyse the microbial flora in dairy cow's vagina,isolate and identify Lactobacillus with strong acid-producing capacity from vagina of healthy dairy cows.Method The standard microbiological methods were used to isolate and identify the bacteria in dairy cow's vagina.Result The most dominant bacteria in the vagina of healthy dairy cows belonged to Lactobacillus(P<0.01);The most dominant bacteria in the vagina of infertile dairy cows belonged to Staphylococcus aureus(P<0.01);55 lactic acid bacte...
Keywords:Dairy cow's vagina  Microbial flora  Lactobacillus  Isolation and identification  
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