Regeneration of peppermint and orange mint from leaf disks |
| |
Authors: | J M Van Eck S L Kitto |
| |
Institution: | (1) Delaware Agricultural Experiment Station, Department of Plant and Soil Sciences, College of Agricultural Sciences, University of Delaware, 19717-1303 Neward, DE, USA;(2) Present address: Department of Plant Breeding, Cornell Univ., 14853 Ithaca, NY, USA |
| |
Abstract: | Leaf disks from peppermint, spearmint, orange mint, lavender mint and Scotch spearmint were cultured on various Murashige-Skoog-based media in order to regenerate shoots. A significantly larger average number of orange mint leaf disks regenerated shoots on basal medium containing 44.4 M benzyladenine (BA) and 250 ml l-1 coconut water (CW). Shoots regenerated from peppermint leaf disks cultured on basal medium containing 44.4 M BA and 250 ml or 450 ml l-1 CW. The most shoots regenerated from orange mint leaf disks cultured on medium containing 10 g l-1 washed Difco Bacto-agar. Disks excised from the bases of the first expanding pair of orange mint leaves cultured under dark conditions regenerated a significantly larger average number of shoots. Histological studies suggested that shoots regenerated from the palisade parenchyma cells associated with vascular tissue.Abbreviations BA
benzyladenine
- NAA
1-naphthaleneacetic acid
- TIBA
2,3,5-triiodobenzoic acid
- CW
coconut water |
| |
Keywords: | Lamiaceae Mentha organogenesis tissue culture |
本文献已被 SpringerLink 等数据库收录! |
|