Barley necrotic locus nec1 encodes the cyclic nucleotide-gated ion channel 4 homologous to the Arabidopsis HLM1 |
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Authors: | Nils Rostoks Deric Schmierer Sharon Mudie Thomas Drader Robert Brueggeman David G Caldwell Robbie Waugh Andris Kleinhofs |
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Institution: | (1) Scottish Crop Research Institute, Genome Dynamics, Invergowrie, DD2 5DA Dundee, Scotland, UK;(2) Department of Crop and Soil Sciences, Washington State University, Pullman, WA 99164, USA;(3) School of Molecular Biosciences, Washington State University, Pullman, WA 99164, USA |
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Abstract: | Barley homolog of the Arabidopsis necrotic (disease lesion mimic) mutant HLM1 that encodes the cyclic nucleotide-gated ion channel 4 was cloned. Barley gene was mapped genetically to the known necrotic
locus nec1 and subsequent sequence analysis identified mutations in five available nec1 alleles confirming barley homolog of Arabidopsis HLM1 as the NEC1 gene. Two fast neutron (FN) induced mutants had extensive deletions in the gene, while two previously described nec1 alleles had either a STOP codon in exon 1 or a MITE insertion in intron 2 which caused alternative splicing, frame shift
and production of a predicted non-functional protein. The MITE insertion was consistent with the reported spontaneous origin
of the nec1 Parkland allele. The third FN mutant had a point mutation in the coding sequence which resulted in an amino acid change in
the conserved predicted cyclic nucleotide-gated ion channel pore region. The expression of two pathogenesis-related genes,
HvPR-1a and β-1,3-glucanase, was elevated in two FN necrotic lines. Ten other members of the barley cyclic nucleotide-gated ion channel
gene family were identified and their position on barley linkage map is reported.
Electronic Supplementary Material Supplementary material is available for this article at and is accessible for authorized users. |
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Keywords: | NEC1 HLM1 Cyclic nucleotide-gated ion channel Necrotic mutant Disease lesion mimic mutant |
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