3α-Hydroxysteroid Oxidoreductase in Rat Brain |
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| Authors: | Neil R Krieger Robert G Scott |
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| Institution: | Departments of Pharmacology and Psychiatry, University of Pennsylvania, Philadelphia, Pennsylvania, U.S.A. |
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| Abstract: | Abstract: We describe a simple procedure for the microassay of 3α-hydroxysteroid oxidoreductase in homogenates of rat brain. This enzyme converts dihydrotestosterone to 3α-androstandiol. We have mapped the distribution of the enzymatic activity in 14 regions of the rat brain. The highest activities were observed in homogenates of olfactory bulb (51/nmol/mg protein/h) and olfactory tubercle (29 nmol/mg protein/h). Substantially lower values were seen in the other brain regions, including thalamus, caudate nucleus, frontal cortex, hippocampus, hypothalamus, and preoptic area (6–20 nmol/mg protein/ h). |
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| Keywords: | 3α-Hydroxysteroid oxidoreductase 3α-Androstandiol Testosterone Dihydrotestosterone Olfactory bulb |
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