An improved procedure for background correction in autoradiography |
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Authors: | H. Korr H. Schmidt |
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Affiliation: | (1) Abteilung Anatomie der RWTH Aachen, Melatener Strasse 211, D-5100 Aachen, Federal Republic of Germany |
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Abstract: | Summary In the event of weak autoradiographic labelling, the proportion of truly labelled cells or structures can be calculated from the frequency distributions of grains per area or cell structure fori=0, 1,...,n grains using the results obtained for an experimental group after the application of a radioactively labelled substance and those obtained for a control group without radioactivity. The principle of this computer-aided method is also applicable when the grain counts are related to varying areas in histological sections.Dedicated to Professor Dr. T.H.Schiebler on the occasion of his 65th birthday |
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