| Abstract: | In this paper we have investigated nonenzimatic hydrolysis of ATP stimulated by calixarene C-107. It has been shown the dependences of the kinetic characteristics from reagent concentration: the maximal value released Pi did not depend on ATP concentration and linearly increased with the growth of calixarene concentration. Besides the growth concentration of ATP or calixarene increased the maximum instantaneous velocity of the reaction and decreased characteristic time. It was identified that univalent cation of Na+, K+, Li+, choline+ and bivalent cation of Ca2+ or Mg2+ did not influence the reaction of ATP hydrolysis, in the presence of other bivalent cation the inhibition of the reaction occurred in line with the sequence: Cu2+ > Ba2+ > Pb2+ > Sr2+ > Ni2+ = Zn2+ > Mn2+ > > Co2+. The alkalization in the range of pH 6.0-8.0 stimulated the ATP hydrolysis. The magnitude of activation energy of the reaction was 50.7 +/- 8.9 kilojoules per mole. The specificity for nucleoside tri- and di-phosphates was not observed. Obtained data can be useful for designing the synthetic ATP-hydrolyzing catalysts and also for subsequent investigation of kinetics, energetics and mechanism of both enzymatic and nonenzymatic ATP hydrolysis reaction. |
