Site-specific recombination events mediated by the DNA invertase Cin of bacteriophage P1 during transformation |
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Authors: | Shigeru Iida |
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Institution: | Department of Microbiology, Biozentrum, University of Basel, Klingelbergstrasse 70, CH-4056 Basel, Switzerland |
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Abstract: | Abstract Bacteriophage P1 encodes the site-specific recombinase Cin which promotes inversion of the C segment, thus controlling the P1 host range. Cin can also mediate inefficient inversion between the normal crossover site cixL and a quasi-crossover site cixQ 1 in inverted orientation. Inversion between cixL and cixQ 1 occurs more frequently in a short period of time after transformation with a plasmid carrying the cin gene, cixL and cixQ 1 than in an established transformant of the plasmid. This is also the case for Cin-mediated deletion on a plasmid containing the cin gene and directly repeated cix sites. |
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Keywords: | Site-specific recombination DNA inversion deletion transformation bacteriophage P1 |
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