A preliminary investigation of the Nostoc punctiforme proteome |
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Authors: | Hunsucker Stephen W Klage Karsten Slaughter Stephen M Potts Malcolm Helm Richard F |
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Affiliation: | Department of Biochemistry, Virginia Tech, Blacksburg, VA 24061, USA. |
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Abstract: | Nostoc punctiforme ATCC 29133 is a filamentous terrestrial cyanobacterium (prokaryote) that expresses several different phenotypes in response to environmental cues. When grown in nitrogen-deficient media the most abundant proteins in addition to phycobiliproteins were superoxide dismutase, ATP synthase, and peptidyl-prolyl cis-trans isomerases. A methylated peptide from an akinete marker protein was also identified, suggesting that methylation could potentially play a regulatory role through signaling. C-phycocyanin alpha-chain was methylated at the C-terminal end of the protein and tandem mass spectrometric data also identified peptides that were deamidated. Since a significant number of putative polyketide/non-ribosomal peptide synthase genes are present in the annotated genome, an analysis of a methanolic extract of whole cells was also performed, and a series of nostopeptolides were identified. |
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Keywords: | Deamidation Electrophoresis Mass spectrometry Methylation Nostoc punctiforme Nostopeptolides Proteome |
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