Proteome profile and biological activity of caprine, bovine and human milk fat globules |
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Authors: | Spertino Stefano Cipriani Valentina De Angelis Chiara Giuffrida Maria Gabriella Marsano Francesco Cavaletto Maria |
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Institution: | Department of Environmental and Life Sciences, University of Piemonte Orientale, Viale T. Michel, 11, 15121 Alessandria, Italy. |
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Abstract: | Upon combining bidimensional electrophoresis with monodimensional separation, a more comprehensive analysis of the milk fat globule membrane has been obtained. The proteomic profile of caprine milk fat globules revealed the presence of butyrophilin, lactadherin and perilipin as the major proteins, they were also associated to bovine and human milk fat globule membranes. Xanthine dehydrogenase/oxidase has been detected only in monodimensional gels. Biological activity of milk fat globules has been evaluated in Caco2-cells, as a representative model of the intestinal barrier. The increase of cell viability was indicative of a potential nutraceutical role for the whole milk fat globule, suggesting a possible employment in milk formula preparation. |
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