Sexual process in eight-spored ascus-forming yeast, Schizosaccharomyces japonicus I. Culture medium for the synchronous sexual process |
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Authors: | Tsuboi Michio; Ohashi Kunihiro; Takahara Michio; Hayashibe Masaya |
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Institution: | Department of Biology, Faculty of Science, Osaka City University Sumiyoshi-ku, Osaka 558, Japan |
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Abstract: | The sexual process of Schizosaccharomyces japonicus consistsof sexual flocculation, zygote formation, eight-spored ascusformation and liberation of spores from the ascus. The culturemedium in which this sexual process took place synchronouslywas prepared. For the completion of the sexual process, glucosewas essential and inorganic salts and vitamins were also required.Elimination of the nitrogen source stimulated the rate of sporeformation. The temporal relationship among the sexual eventswas also elucidated: sexual flocculation, zygote formation,ascus formation and spore liberation occurred at 4, 7, 13 and20 hr, respectively, after transfer to the medium for the synchronoussexual process. (Received May 11, 1978; ) |
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