The isolation of barley-aleurone protoplasts |
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| Authors: | Lincoln Taiz Russell L Jones |
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| Institution: | (1) Department of Botany, University of California, Berkeley |
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| Abstract: | Summary A procedure is described for the isolation of large numbers of viable aleurone protoplasts. After treatment with  Onozuka cellulase protoplasts were obtained which were surrounded by a thin, Onozuka-resistant wall. These cells were termed spheroplasts. Treatment of spheroplasts with Glusulase digested away the residual wall, yielding naked protoplasts. Measurements of respiration using a Clarke-type oxygen electrode indicated that aleurone cells isolated by this procedure were viable.Work supported by National Science Foundation grant GB-27468. |
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