Pteroylpolyglutamate synthesis by lung- and culture-derived Pneumocystis carinii |
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Authors: | Yu-long Hong Marilyn S. Bartlett Sherry Queener James W. Smith Margaret Shaw Steven R. Meshnick |
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Affiliation: | Department of Molecular Life Science, Tokai University School of Medicine, Isehara 259-11, Japan; College of Medical Care and Technology, Gunma University, Maebashi 371, Japan |
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Abstract: | Abstract Evaluation of four β-lactamase inhibitors in terms of their outer membrane permeability in Pseudomonas aeruginosa revealed that sulbactam and tazobactam diffused most efficiently and equally well. That of BRL42715 appeared to be a factor of ten lower than that of the above two, but it showed the strongest β-lactamase inhibitory activity. This is most likely due to its better β-lactamase inactivating activity. BRL42715 at 1.56 μg ml−1 lowered the minimum inhibitory concentrations of ceftazidime and imipenem in a strain producing fully derepressed β-lactamase and an undetectable level of the outer membrane protein OprD2. |
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Keywords: | Outer membrane Membrane permeability β-Lactamase inhibitor Pseudomonas aeruginosa |
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