Angiotensinogen,angiotensine converting enzyme and plasminogen activator inhibitor-1 gene polymorphism in chronic allograft dysfunction |
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Authors: | Negar Azarpira M Bagheri Gh A Raisjalali M H Aghdaie S Behzadi H Salahi M Rahsaz M Darai M J Ashraf B Geramizadeh |
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Institution: | (1) Organ Transplant Research Center, Nemazi Hospital, Shiraz University of Medical Sciences, Zand street, Shiraz, 7193711351, Iran;(2) Transplant Center, Shiraz University of Medical Sciences, Shiraz, Iran |
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Abstract: | Despite dramatic improvements in first-year patient and graft survival rates, chronic allograft dysfunction (CAD) remains
the leading cause of late renal allograft loss, while current immunologic strategies have little effect on this condition.
The renin–angiotensin system (RAS) plays an important role in progression of chronic renal disease. It was shown that plasminogen
activator inhibitor-1 (PAI-1) functions in the RAS. This study investigates the possible links between angiotensinogen (AGT
M235T), angiotensin-converting enzyme (ACE) and PAI-1 genotypes with CAD. Assessments of polymorphism were performed in 127
renal allograft recipients (77 with CAD and 50 with normal renal function). Fifty healthy subjects were also considered for
comparison. Genotypes were determined using polymerase chain reaction (PCR) sequence-specific primers and PCR followed by
restriction fragment length polymorphism analysis. Kidney recipients with CAD had significantly higher frequencies of the
TT than the recipients without CAD (P < 0.05). The transplant recipients with CAD also had significantly higher frequencies of the DD genotype than those without
CAD (P < 0.05). No significant differences were observed between the allelic and genotypic distributions of PAI-1 polymorphisms.
Therefore, determination of AGT M235T and ACE genotypes prior to transplantation may be useful to identify patients who are
at risk for chronic renal transplant dysfunction. |
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