| 产角蛋白酶耐热金孢菌的初筛研究 |
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| 引用本文： | 梁建东,韩燕峰,梁宗琦,张纪伟. 产角蛋白酶耐热金孢菌的初筛研究[J]. 菌物研究, 2007, 5(4): 210-213 |
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| 作者姓名： | 梁建东 韩燕峰 梁宗琦 张纪伟 |
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| 作者单位： | 贵州大学生命科学学院真菌资源研究所,贵阳,550025 |
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| 基金项目： | 国家自然科学基金重大项目子项目(30499340) |
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| 摘 要： | 用鸡羽毛粉或人头发粉作为唯一碳、氮源,对9个耐热的金孢属(Chrysosporium spp.)真菌菌株进行了产角蛋白酶筛选。研究结果表明:菌株H-49-2对鸡羽毛的利用能力最强,而菌株H-143-1对人头发的利用能力最强。对H-49-2菌株进行液体发酵产酶试验的结果表明,培养6d时酶活性最大,为9.6U/mL。
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| 关 键 词： | 金孢属 角蛋白酶 耐热真菌 |
| 文章编号： | 1672-3538(2007)04-0210-04 |
| 修稿时间： | 2007-09-03 |
Primary Screening of Keratinase-Producing Strains of Thermotolerant Chrysosporium |
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| LIANG Jian-dong,HAN Yan-feng,LIANG Zong-qi,ZHANG Ji-wei. Primary Screening of Keratinase-Producing Strains of Thermotolerant Chrysosporium[J]. Journal of Fungal Research, 2007, 5(4): 210-213 |
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| Authors: | LIANG Jian-dong HAN Yan-feng LIANG Zong-qi ZHANG Ji-wei |
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| Abstract: | Nine themotolerant strains of Chrysosporium were screened with chicken feather or human hair powder as the sole carbon and nitrogen source for keratinase production.The results showed that strain H-49-2 had the strongest ability to degrade chicken feather powder,while strain H-143-1 had the strongest ability to degrade human hair powder.According to liquid fermentation of the H-49-2 strain,the keratinase activity reached a highest level on the 5th day,up to 9.6 U/mL;as the sole source of carbon and nitrogen,chicken feather powder contributed to keratinase production while the addition of glucose or plus peptonerestrained keratinase production. |
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| Keywords: | Chrysosporium keratinase themotolerant fungi |
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