| 一种新的测定蛋白激酶活性的方法──毛细管电泳测定蛋白激酶A的活性 |
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| 引用本文： | 万谦,陈长征,李伯良,夏其昌.一种新的测定蛋白激酶活性的方法──毛细管电泳测定蛋白激酶A的活性[J].生物化学与生物物理进展,1994,21(3):267-267. |
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| 作者姓名： | 万谦 陈长征 李伯良 夏其昌 |
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| 作者单位： | 中国科学院上海生物化学研究所;中国科学院上海生物化学研究所;中国科学院上海生物化学研究所;中国科学院上海生物化学研究所 |
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| 基金项目： | “863”计划资助项目. |
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| 摘 要： | 建立了以毛细管电泳为基础的测定蛋白激酶A活性的新方法，可作为激酶测活的通用方法．此法基于底物及其磷酸化产物很容易在毛细管电泳中分开，且酶活力可用积分值计算，同时又发展了连续进样技术，能在一次电泳中同时进行10个以上的酶活性测定，新方法操作简单，灵敏度和精确性均优于常规的同位素法．
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| 关 键 词： | 蛋白激酶A活力 磷酸化 毛细管电泳 |
| 收稿时间： | 1994/1/11 0:00:00 |
| 修稿时间： | 1994/2/15 0:00:00 |
A New Assay for Kinase Activity: The Determination of Protein Kinase Using Capillary Electrophoresis |
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| Wan Qian,Chen Changzheng,Li Boliang and Xia Qichang.A New Assay for Kinase Activity: The Determination of Protein Kinase Using Capillary Electrophoresis[J].Progress In Biochemistry and Biophysics,1994,21(3):267-267. |
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| Authors: | Wan Qian Chen Changzheng Li Boliang and Xia Qichang |
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| Institution: | Shanghai Institute of Biochemistry.Academia Sinica;Shanghai Institute of Biochemistry.Academia Sinica;Shanghai Institute of Biochemistry.Academia Sinica;Shanghai Institute of Biochemistry.Academia Sinica |
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| Abstract: | A new assay of protein kinase A using capillary electrophoresis has been established.It is a universal and useful method for kinase activity. The method based on principle that substrate (kemptide)and product (phosphorylated kemptide) are easily seperated by capillary electrophoresis and the enzyme activity can be calculated on the integrated area. A continous sampling technique which can analyse more than 10 samples in one run has also been developed.The new method is easy to operate and its accuracy and sensitivity are higher than that of conventional isotopic method. |
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| Keywords: | protein kinase A activity capillary electrophoresis phosphorylation |
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