毛细管电泳测定蛋白激酶A活力的新方法

建立了以毛细管电泳为基础的蛋白激酶Ａ活力测定的新方法，对其他一些激酶的活力测定具有一定的通用性。此方法是基于蛋白激酶Ａ的检测底物及其磷酸化产物容易在毛细管电泳中分开，并且可以通过在线检测进行积分定量。同时发展了连续进样技术，使能在一个电泳过程中分析十个以上的样品，大大节省分析时间和费用。

A New Assay for Protein Kinase A Using Capillary Electrophoresis

A new and novd assay of Protein Kinase A using caplliary eleotrophoresis has been established, it proves to be a general and useful method for other kinases as well.The method is based on the pinciple that the substrate(Kemptide)and the product(phosporylated Kemptide)are easily seperand and quantitated b capillary electrophoresis.It is easy to operate and the result obtained is similar to that of conventional isotopic method.We have also developed a continuous sampling technique which can analyze more than 10 samples in one electrophoretic run and save much analysis time.