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Metabolic activity interferometer: description and calibration of an interferometric method to measure growth of mycobacteria |
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| Authors: | R. R. P. Machado E. S. Lima Filho D. F. Jardim M. A. A. Ferreira C. G. de Faria R. S. Duarte B. Lesche | ||
| Affiliation: | 1.Programa de pós-gradua??o de Biotecnologia Vegetal,Universidade Federal do Rio de Janeiro,Rio de Janeiro,Brazil;2.SUPREMA, Faculdade de Ciências Médicas e da Saúde de Juiz de Fora,Juiz de Fora,Brazil;3.Physics Department,Universidade Federal de Juiz de Fora,Juiz de Fora,Brazil;4.Universidade Vale do Rio Doce,Governador Valadares,Brazil;5.Milk Quality Laboratory,Embrapa Dairy Cattle,Juiz de Fora,Brazil;6.Institute of Microbiology,Federal University of Rio de Janeiro,Rio de Janeiro,Brazil | ||
| Abstract: | An interferometer that measures the refractive index changes due to bacterial metabolism is described. The apparatus permits simultaneous and real time measurement of bacterial growth in several samples of slowly growing mycobacteria. The error sources are discussed and the sensitivity of the apparatus is tested. For the species Mycobacterium bovis BCG and M. smegmatis, a relation between refractive index change and bacterial concentration is determined experimentally and the time constant of bacterial growth is measured.
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| Keywords: | Bacterial growth Tuberculosis Interferometry Refractive index | ||
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