| 家蚕核型多角体病毒蛋白组份检测方法建立 |
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| 引用本文： | 李卫党,季平. 家蚕核型多角体病毒蛋白组份检测方法建立[J]. 微生物学杂志, 1999, 19(3): 5-6 |
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| 作者姓名： | 李卫党 季平 |
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| 作者单位： | 江苏省镇江医学院免疫研究室!2120012(李卫党，李良菊，许化溪，臧磊，刘恭植)，中国农业科学院蚕业研究所(季平，何家禄) |
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| 基金项目： | 863科学基金!102一11－02-06 |
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| 摘 要： | 研制了兔抗家蚕核型多角体病毒蛋白组份的多克隆抗体，用间接法建立了家蚕核型多角体病毒蛋白的dot-ELISA检测方法。此法检测多角体病毒蛋白的灵敏度达10ng，与人血清和兔血清无交叉反应，可用于家蚕生物反应器生产的目的蛋白中多角体病毒蛋白组份的检测。
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| 关 键 词： | dot-ELISA 家蚕核型多角体病毒蛋白组份 抗体 |
METHOD ESTABLISHMENT OF DOT-ELISA FOR PROTEIN COMPONENT DETECTION FROM BOMBYX MOAY NUCLEAR POLYHEDROSIS VIRUS |
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| Li Weidang et al.. METHOD ESTABLISHMENT OF DOT-ELISA FOR PROTEIN COMPONENT DETECTION FROM BOMBYX MOAY NUCLEAR POLYHEDROSIS VIRUS[J]. Journal of Microbiology, 1999, 19(3): 5-6 |
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| Authors: | Li Weidang et al. |
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| Abstract: | Poyclonal antibody to the protein of Bm-NPV was produced by conventional immunization to rabbit.An indirect type of do-ELISA for protein from Bombyx mori nuclear polyhedrosis virus was established.The Sensitivity of the immunoassay was 10ng. No cross-reaction was found with human serum and rabbit serum. This assay could be applied in detecting the leftover protein of the product that Bm-NPV gene expression system produced. |
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| Keywords: | dot-ELISA Bombyx mori nuclear polyhedrosis virus antibody |
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