Sterilization of bioreactor media on the basis of computer calculated thermal input designated asF
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Authors: | LaVerne D Boeck Roger W Wetzel Stanton C Burt Floyd M Huber Gary L Fowler Joseph S Alford Jr |
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Institution: | (1) Eli Lilly and Company, Indianapolis, IN, USA;(2) Lilly Research Laboratories, Lilly Corporate Center, 46285 Indianapolis, IN, USA |
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Abstract: | Summary Industrial fermentation media are normally sterilized with steam to destroy the indigenous microbial population prior to inoculation with a specific microorganism. Because biological validation of each sterilization cycle is impractical, an overkill approach is commonly employed on the basis that alteration of heat-sensitive nutrients is less detrimental than survival of indigenous microbes. However, the heat destruction of microbes is known to be a probability function amenable to calculation. A computer has been programmed to calculate the on-line heat input asF
0 values during sterilization of media in stirred bioreactors. The accumulation ofF
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Keywords: | Sterilization Bioreactor Computer F
0 Voice synthesizer Thermal input |
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