Microbial community structure and ecology of subglacial sediments in two polythermal Svalbard glaciers characterized by epifluorescence microscopy and PLFA |
| |
Authors: | Klára Ka?tovská Marek Stibal Marie ?abacká Barbora ?erná Hana ?antr??ková Josef Elster |
| |
Institution: | (1) Institute of Botany, Academy of Sciences of the Czech Republic, Dukelská 135, Třeboň, 379 82, Czech Republic;(2) Faculty of Biological Sciences, University of South Bohemia, České Budějovice, Czech Republic;(3) Institute of Hydrobiology, Academy of Sciences of the Czech Republic, České Budějovice, Czech Republic |
| |
Abstract: | Biological and physico-chemical characteristics of subglacial sediments were studied in Svalbard. Sediment from close proglacial
and supraglacial environments was used for a comparison. Viable bacteria, cyanobacteria and microalgae were detected in subglacial
sediments from two polythermal glaciers using epifluorescence microscopy and phospholipid fatty acid (PLFA) analyses. The
subglacial samples were generally of higher pH values, coarser texture and lower water content, organic matter, organic carbon,
and nitrogen compared to proglacial and supraglacial sediments). Bacterial counts of 1.6 × 107 cells mg− 1 OM (organic matter) were found. Cyanobacteria and algae were also of low abundance 4.2 cells mg− 1 DW (dry weight)]. Cyanobacteria comprised the major proportion of the autophotothrophic assemblages of subglacial soils.
Deglaciated soils were similar to subglacial sediment in physico-chemical properties and microbial structure and numbers,
unlike soil from vegetated sites or cryoconite sediment. In subglacial and deglaciated soil, relatively low diversity of microorganisms
and low substrate availability was detected by PLFA analyses. Good accordance in microbial community structure assessments
between epifluorescence microscopy and PLFA analyses was found. Our results suggest that the subglacial microbial populations
can be divided into two groups: autochthonous microorganims (chemoheterotrophic bacteria) and allochthonous that retain the
ability to proliferate and give rise to active population when conditions become favorable.
Electronic Supplementary Material Supplementary material is available to authorised users in the online version of this article at . |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|