Pyrethroid-degrading Sphingobium sp. JZ-2 and the purification and characterization of a novel pyrethroid hydrolase |
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| Authors: | Peng Guo Baozhan Wang BaoJian Hang Lian Li Shinawar Waseem Ali Jian He Shunpeng Li |
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| Affiliation: | 1. Centre for Environmental Risk Assessment and Remediation, The University of South Australia, Mawson Lakes, Adelaide, SA 5095, Australia;2. Global Centre for Environmental Remediation, Faculty of Science and Information Technology, The University of Newcastle, Callaghan, NSW 2308, Australia;3. Cooperative Research Centre for Contamination Assessment and Remediation of the Environment (CRC-CARE), The University of Newcastle, Callaghan, NSW 2308, Australia;1. MOE Key Laboratory of Environmental Remediation and Ecosystem Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China;2. Department of Environmental Engineering, Quzhou University, Quzhou 324000, China;3. Laboratory for Teaching in Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China;4. Department of Effect-Directed Analysis, Helmholtz Center for Environmental Research – UFZ, Leipzig 04318, Germany;1. State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, Integrative Microbiology Research Centre, South China Agricultural University, Guangzhou 510642, China;2. Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China;3. Department of Water Supply, Sanitation and Environmental Engineering, IHE Delft Institute of Water Education, Institute of Water Education Westvest 7, 2601DA Delft, The Netherlands;1. State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, Integrative Microbiology Research Centre, South China Agricultural University, Guangzhou 510642, China;2. Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China;3. Department of Environmental Engineering and Water Technology, IHE Delft Institute for Water Education, Westvest 7, 2601DA Delft, the Netherlands;4. School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, China;1. School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou, 510006, People’s Republic of China;2. Department of Chemistry, Liverpool School of Tropical Medicine, L3 5QA, Liverpool, United Kingdom;3. Faculty of Biotechnology and Health, Wuyi University, Jiangmen, 529020, People’s Republic of China;1. School of Food and Biological Engineering, Zhengzhou University of Light Industry, 5 Dongfeng Rd, Zhengzhou 450002, PR China;2. State Key Laboratory of Microbial Technology, Shandong University, 27 Shanda Nan Rd, Jinan 250100, PR China |
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| Abstract: | A pyrethroid-degrading bacterium strain JZ-2 was isolated from activated sludge treating pyrethroid-manufacturing wastewater. Based on the morphological, physiological and biochemical characterization, and phylogenetic analysis of the 16S rRNA gene sequence, the strain was identified as Sphingobium sp. Strain JZ-2 was capable of degrading fenpropathrin, cypermethrin, permethrin, cyhalothrin, deltamethrin, fenvalerate and bifenthrin. This strain degraded fenpropathrin by hydrolysis of the carboxylester linkage to yield 3-phenoxybenzaldehyde and 2,2,3,3-tetramethylcyclopropanecarboxylic acid. 3-Phenoxybenzaldehyde, 3-phenoxybenzoate, protocatechuate and catechol are the intermediates of fenpropathrin degradation. Protocatechuate and catechol were further oxidized by ortho-cleavage pathway. A novel pyrethroid hydrolase from cell-free extract was purified 108.5-fold to apparent homogeneity with a 10.2% overall recovery. It was a monomer with a molecular mass of 31 ± 1 kDa, a pI of 4.85. The optimal pH and temperature were 7.5 and 40 °C, respectively. No cofactors or coenzymes were required for the pyrethroid-hydrolysis activity. The enzyme was strongly inhibited by many irons (Ag+, Cu2+, Hg2+ and Zn2+), SDS, p-chloromercuribenzoic acid, phenylmethylsulfonyl fluoride and malathion. |
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