Characterization of biosurfactant produced by Pseudoxanthomonas sp. PNK-04 and its application in bioremediation |
| |
| Authors: | Anand S. Nayak M.H. Vijaykumar T.B. Karegoudar |
| |
| Affiliation: | 1. Institute of Chemical Technology and Engineering, Poznan University of Technology, Pl. M. Sk?odowskiej-Curie 2, 60-965 Poznań, Poland;2. Institute of Food Technology of Plant Origin, Poznań University of Life Sciences, Wojska Polskiego 31, 60-624 Poznań, Poland;3. Department of Biotechnology and Food Microbiology, University of Life Sciences in Poznań, Wojska Polskiego 48, 60-627 Poznań, Poland;1. Centre for the Environment, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India;2. Department of Chemical Engineering, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India;3. Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India;1. Key Laboratory of Beijing for Water Quality Science and Water Environmental Recovery Engineering, College of Civil Engineering, Beijing University of Technology, Beijing 100124, China;2. State Nuclear Electric Power Planning Design & Research Institute, Beijing 100085, China;1. School of Civil & Engineering, National International Cooperation Based on Environment and Energy, University of Science and Technology Beijing, 30 Xueyuan Road, Haidian District, 100083 Beijing, PR China;2. Key Laboratory of Metal and Mine Efficiently Exploiting and Safety of Chinese Ministry of Education, University of Science and Technology Beijing, 30 Xueyuan Road, Haidian District, 100083 Beijing, PR China;3. State Key Laboratory of Biogeology and Environmental Geology of Chinese Ministry of Education, China University of Geoscience, Wuhan 430074, PR China;4. Sino-Hungarian Joint Laboratory of Environmental Science and Health, School of Environmental Studies, China University of Geoscience, Wuhan 430074, PR China;5. Department of Botany, Faculty of Science, University of Ruhuna, Matara, Sri Lanka;6. Domain Expert, Petroleum Microbiology and Unconventional Oil/Gas, Oil and Natural Gas Corporation Ltd., Dehradun, India;7. Department of Chemistry, Hong Kong Baptist University, 224 Waterloo Road, Kowloon Tong, Hong Kong Special Administrative Region, China;1. School of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, PR China;2. School of Environment and Energy, South China University of Technology, Guangzhou 510006, PR China;3. State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 51006, PR China;4. River Basin Research Center, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan |
| |
| Abstract: | Biosurfactant producing bacterium was identified as Pseudoxanthomonas sp. PNK-04 based on morphological, physiological, biochemical tests and 16S rRNA gene sequencing. This strain was screened for biosurfactant production using different carbon sources by measuring the surface tension of the medium at different time intervals, and hemolytic activity. The produced biosurfactant was found to be a rhamnolipid based on the formation of dark blue haloes around the colonies in CTAB–methylene blue agar plates and the content of rhamnose sugar. The rhamnolipids produced by this bacterium were found to contain mono- and dirhamnose units linked to β-hydroxy alkonic acids containing 8–12 carbon atoms. This biosurfactant has high emulsifying activity when compared to chemical surfactants such as Tween-80 and Triton X-100 with respect to aliphatic and aromatic hydrocarbons. Further, the biosurfactant stimulates the degradation of 2-chlorobenzoic acid, 3-chlorobenzoic acid and 1-methyl naphthalene by Pseudoxanthomonas sp. PNK-04 probably by aiding in the uptake and increasing the solubility. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
