Crucial factors affecting the nitrile hydratase production of Mesorhizobium sp. F28 |
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| Authors: | Yun-Shu Feng Chi-Mei Lee |
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| Institution: | 1. Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350116, China;2. QuanZhou Women''s and Children''s Hospital, China;3. State Key Laboratory of Environmental and Biological Analysis, Department of Chemistry, Hong Kong Baptist University, Hong Kong, China;1. Key Laboratory of Bio-organic Fertilizer Creation, Ministry of Agriculture, Institute for Applied Microbiology, Anhui Science and Technology University, Bengbu 233100, China;2. School of Medicine, Zhejiang University, Hangzhou 310058, China;3. Department of Molecular and Cell Biology, Tongji University, Shanghai 200092, China;1. Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939-0398, Japan;2. Asano Active Enzyme Molecule Project, ERATO, JST, 5180 Kurokawa, Imizu, Toyama 939-0398, Japan;1. INTECH (Instituto Tecnológico Chascomús), CCT-La Plata, CONICET - Universidad Nacional de San Martín, Avenida Intendente Marino, Km 8.2, 7130 Chascomús, Argentina;2. IBBM (Instituto de Biotecnología y Biología Molecular), CCT-La Plata, CONICET, Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, 1900 La Plata, Argentina;3. CREG (Centro Regional de Estudios Genómicos), CONICET - Universidad Nacional de La Plata, 1900 La Plata, Argentina;4. Section of Microbial Ecology and Biotechnology, Department of Plant and Environmental Sciences, University of Copenhagen, 1871 Frederiksberg, Denmark |
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| Abstract: | Mesorhizobium sp. F28 contains cobalt-NHase, which effectively converts acrylonitrile into acrylamide. When urea was added to the culture medium, the NHase activity was 62.3 U ml?1 (R2A–R2A/urea) after 22.5 h of cultivation, which was similar to that in the medium without addition (R2A–R2A, 70.0 U ml?1). The relative activity of the purified NHase was 100%, 92%, 94%, and 92% in the medium containing, respectively, 0 mM, 2 mM, 5 mM, and 10 mM of urea. Urea had no significant effect on the purified NHase activity of Mesorhizobium sp. F28. This research did not observe the NHase production by Mesorhizobium sp. F28 when acrylonitrile was supplemented in the culture medium except that cobalt ions existed. The highest enzyme activity was 328.5 U ml?1 as cobalt ions were added in the pre-culture and culture medium after 22.5 h of cultivation (R2A/Co-R2A/Co); compared to media without cobalt ions (R2A–R2A, 22.5 h, 70.5 U ml?1) this is an almost five-fold enhancement. It can be concluded that culture media containing cobalt ions was beneficial for the formation of active NHase of Mesorhizobium sp. F28. |
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