Degradation of the multiple branched alkane 2,6,10,14-tetramethyl-pentadecane (pristane) in Rhodococcus ruber and Mycobacterium neoaurum |
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| Authors: | Le Thi Nhi-Cong Annett Mikolasch Hans-Peter Klenk Frieder Schauer |
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| Institution: | 1. Ernst-Moritz-Arndt-University Greifswald, Institute for Microbiology, F.L. Jahnstr. 15A, 17487 Greifswald, Germany;2. Department of Microbiology, DSMZ – German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124 Braunschweig, Germany;1. Regional University Center of Excellence in Environmental Industry, Szent István University, Gödöll?, Hungary;2. Department of Environmental Protection and Environmental Safety, Szent István University, Gödöll?, Hungary;3. Bioengineering Department, Sapientia Hungarian University of Transylvania, Miercurea Ciuc, Romania;4. Department of Microbiology, Eötvös Loránd University, Budapest, Hungary;5. Department of Applied and Environmental Microbiology, Research Institute of Agro-Environmental Sciences, National Agricultural Research and Innovation Centre, Budapest, Hungary;1. Department of Chemical Engineering, Shahid Bahonar University of Kerman, Iran;2. School of Chemical and Petroleum Engineering, Sharif University of Technology, Iran;3. Institute of Biotechnology, Shiraz University, Iran;1. Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran;2. Dermatology and Leishmaniasis Research Center, Department of Parasitology, School of Medicine, Kerman University of Medical Sciences, End of 22nd Bahman BLVD, Kerman 7616714115, Iran;3. Department of Microbiology, Science and Research Branch, Islamic Azad University, Kerman, Iran;4. Department of Microbiology, Islamic Azad University, Kerman, Iran;1. Department of Pharmacy and Biotechnology, University of Bologna, Via Irnerio 42, 40126 Bologna, Italy;2. Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126 Milano, Italy;3. CNR, Institute for Biomedical Technologies, Via Fratelli Cervi 93, 20090 Segrate, Milan, Italy;4. Department of Agrifood Production and Environmental Sciences, University of Firenze, Piazzale delle Cascine 24, 50144 Florence, Italy |
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| Abstract: | Pristane, a highly branched hydrocarbon that also contains iso-branched termini, was used as a substrate for several alkane-metabolizing bacteria. Rhodococcus ruber and Mycobacterium neoaurum were able to utilize pristane for growth effectively. The intermediates produced by these bacteria during incubation with pristane were analyzed by gas chromatography (GC) and gas chromatography/mass spectra (GC/MS). The products revealed as products of 4-methyl pentanoic acid; methyl butanedioic acid; 2-methyl pentadioic acid; methyl propanedioic acid; 4-methyl heptanedioic acid; and 2,6,10,14-tetramethyl-pentadecan-3-one were detected in M. neoaurum cultures. In R. ruber, methyl butanedioic acid; 2-methyl pentadioic acid; 4,8-dimethylnonanoic acid, 4-methyl heptanedioic acid; 2,6,10-trimethylundecanoic acid; 3,7-dimethyl decanedioic acid; and 2,6,10,14-tetramethyl-pentadecan-3-one were detected. The occurrence of these intermediates showed that pristane could be catabolized not only via mono- but also by a di-terminal oxidation pathway. Furthermore, the presence of 2,6,10,14-tetramethyl-pentadecan-3-one; 3,7-dimethyldecandioate; and 2-methylbutandioate established a third pathway initiated by sub-terminal oxidation at the third carbon atom of pristane. Novel intermediates detected suggest simultaneous sub-terminal and di-terminal oxidation pathways. |
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