Accelerated laboratory test to study fungal biodeterioration of cementitious matrix |
| |
| Authors: | V Wiktor F De Leo C Urzì R Guyonnet Ph Grosseau E Garcia-Diaz |
| |
| Institution: | 1. Delft University of Technology, Faculty of Civil Engineering and Geosciences, P.O Box 5048, 2600GA Delft, The Netherlands;2. Department of Life Sciences, Sect. of Microbiological, Genetic and Molecular Sciences, University of Messina, Salita Sperone 31, 98166 Messina, Italy;3. Department Poudres et Matériaux Multi-Composants, Ecole Nationale Supérieure des Mines de Saint-Etienne, 158 Cours Fauriel, 42023 Saint-Etienne Cedex 02, France;4. Centre des Matériaux de Grande Diffusion, Ecole des Mines d''Alès, 6 avenue de Clavières, 30319 Alès Cedex, France;1. Federal Institute for Materials Research and Testing, Bundesanstalt für Materialforschung und – prüfung BAM, Department 4 Materials & Environment, Unter den Eichen 87, 12205 Berlin, Germany;2. Free University of Berlin, Department of Biology, Chemistry & Pharmacy and Department of Earth Sciences, Malteserstrasse 74-100, 12249 Berlin, Germany;3. British Columbia Institute of Technology, 3700 Willingdon Avenue, Burnaby, BC, Canada V5G 3H2;4. University of British Columbia, 2329 West Mall, Vancouver, BC, Canada V6T 1Z4;5. University of Geneva, LBMPS, Science III, 30 quai Ernest-Ansermet, 1211, Genève 4, Switzerland;6. CBS-KNAW Fungal Biodiversity Centre, P.O. Box 85167, 3508 AD Utrecht, The Netherlands;7. Division of Genomics & Microbial Diversity, Life Science Department, The Natural History Museum, Cromwell Road, London SW7 5BD, UK;1. Mines Douai, LGCgE-GCE, 941 rue Charles Bourseul, 59508 Douai, France;2. Université de Lille 1, Cité Scientifique, 59650 Villeneuve-d''Ascq, France;1. Escola Politécnica da Universidade de São Paulo, Dept. de Engenharia Civil, Av. Prof. Almeida Prado, trav. 2, n.83, Edif. de Eng. Civil, Cidade Universitária, São Paulo SP, CEP 05508-900, Brazil;2. Magnel Laboratory for Concrete Research, Faculty of Engineering and Architecture, Ghent University, Technologiepark Zwijnaarde 904, B-9052, Gent, Belgium;3. University of Oklahoma, Department of Microbiology and Plant Biology, 770 Van Vleet Oval, Norman, OK 73019, USA;1. Cement Research Center, Iran University of Science and Technology, Tehran 1684613114, Iran;2. Biotechnology Research Laboratory, School of Chemical Engineering, Iran University of Science and Technology, Tehran 1684613114, Iran;1. University of Bologna, Ravenna Campus, Via Guaccimanni, 42, 48121 Ravenna, Italy;2. Opificio delle Pietre Dure, viale Filippo Strozzi 1, 50129 Firenze, Italy;3. Department of Biology, University of Florence, Via Madonna del Piano 6, 50019 Sesto Fiorentino, Firenze, Italy;4. Institute for the Conservation and Valorization of Cultural Heritage, National Research Council, Via Madonna del Piano 10, Sesto Fiorentino I-50019, Italy |
| |
| Abstract: | The present study aimed to develop an accelerated laboratory test to study the biodeteriorative effect of different fungal strains to a cementitious matrix. The test developed in this study permits to obtain a rapid fungal development on cement specimens. Three months of experiments only are needed to obtain first results, which is rather shorter than other test developed to date to study fungal biodeterioration. Results are mainly related to aesthetical biodeterioration. Results show that in these experimental conditions, fungal growth occurs since the first week of incubation. Stereomicroscopy observations showed that microbial growth was noticed only on the surface of specimens, while PAS staining revealed the real extent of microbial growth on and within the matrix as later confirmed by SEM observations of cross section showing the penetration of hyphae inside the matrix. Test can be used with short time of incubation, to test and to compare bioreceptivity of cement-based materials; and several months of incubation should allow the study of mechanisms involved in biodeterioration. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
