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Investigations on ultraviolet light and nitrous acid induced mutations of halotolerant bacterial strains for the treatment of tannery soak liquor
Authors:Sudharshan Sekar  Senthilkumar Sivaprakasam  Surianarayanan Mahadevan
Institution:1. Université François Rabelais, Tours, France;2. CHRU de Tours–Hôpital Trousseau, Service de Dermatologie, Tours, France;3. Unité Mixte de Recherche INRA-Univerity of Tours N°1282, Tours, France;4. CHRU de Tours–Hôpital Trousseau, Service d?Anatomie et Cytologie Pathologiques, Tours, France;1. Department of Chemistry, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad 91775-1436, Iran;2. Department of Chemistry, Payame Noor University (PNU), 19395-4697 Tehran, Iran;3. Department of Chemistry, Faculty of Science, Shahid-Bahonar University of Kerman, Kerman, Iran;4. Department of Chemistry, Adam Mickiewicz University, Umultowska 89b, 61-614 Poznań, Poland;5. Molecular Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran;6. Department of Biology, Faculty of Basic Sciences, Hakim Sabzevari University, Sabzevar, Iran;1. RWTH Aachen University, Worringerweg 1, D-52074 Aachen, Germany;2. School of Engineering and Science, Jacobs University Bremen, Campus Ring 1, 28759 Bremen, Germany;3. Molzym GmbH & Co. KG, Mary-Astell-Strasse 10, 28359 Bremen, Germany;1. National Engineering Laboratory for Clean Technology of Leather Manufacture, Sichuan University, Chengdu, 610065, PR China;2. Key Laboratory of Leather Chemistry and Engineering (Sichuan University), Ministry of Education, Chengdu, 610065, PR China;1. Centre for Chemistry and Biotechnology, Deakin University, Waurn Ponds, 75 Pigdons Road, Locked Bag 20000, Geelong, VIC 3220, Australia;2. Centre for Molecular and Medical Research, School of Medicine, Deakin University, Waurn Ponds, 75 Pigdons Road, Locked Bag 20000, Geelong, VIC 3220, Australia;3. Centre for Marine Bioproducts Development, College of Medicine and Public Health, Flinders University, Sturt Road, Bedford Park, Adelaide, SA 5042, Australia
Abstract:The objective of this research work is to study the effect of physical and chemical mutagenesis on biological treatment of tannery saline wastewater (soak liquor) employing halotolerant bacterial strains. Four halotolerant bacterial strains isolated from saline sources were used. The strains were identified as Pseudomonas aeruginosa, Bacillus flexus, Exiguobacterium homiense and Staphylococcus aureus, respectively. The isolates were found to grow well in medium containing 0–10% NaCl. At high saline concentration (>5%), the identified strains and their mixed consortia showed a low degrading efficiency of soak liquor (35–45%). UV light and nitrous acid mutagenesis were performed over the strains and the mutated strains were employed for degradation of soak liquor at high salinity level (6% by wt). Comparison of Chemical Oxygen Demand (COD) removal rates for both pure mutant isolates and mixed mutated consortia showed that nitrous acid mutagenesis resulted in degradation of 71% COD removal. Ultraviolet (UV) mutagenesis has no effect on degradation effectiveness. Biomass sludge (Mixed Liquor Volatile Suspended Solids) growth was also found to be high in nitrous acid treated strains.
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