Isolation of a biphenyl-degrading bacterium,Achromobacter sp. BP3, and cloning of the bph gene cluster |
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| Authors: | Qing Hong Xiaojun Dong Lijuan He Xin Jiang Shunpeng Li |
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| Affiliation: | 1. College of Geography and Environmental Science, Zhejiang Normal University, Jinhua 321004, China;2. Department of Civil and Environmental Engineering, Shantou University, Shantou 515063, China;3. Department of Meteorology, COMSATS University, Islamabad, 44000, Pakistan;4. Environmental Science Research and Design Institute of Zhejiang Province, Hangzhou 310007, China |
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| Abstract: | A bacterial strain, BP3, capable of degrading biphenyl, was isolated from petroleum-contaminated soil. Strain BP3 was identified preliminarily as Achromobacter sp. based on its physiological and biochemical characteristics and 16S rRNA gene sequence analysis. Strain BP3 was able to degrade 50 mg l?1 of biphenyl within 12 h. A 16.7-kb DNA fragment consisting of the entire bph cluster (bphRA1A2XA3A4BCKHJID) was obtained by normal PCR amplification and chromosome walking. Genes encoding integrase and transposon related genes were detected upstream and downstream of the bph cluster, respectively, which indicated that the bph cluster might locate on a big mobile genetic element (MGE). |
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