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Synthetic substrate for application in both high and low throughput assays for botulinum neurotoxin B protease inhibitors |
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| Authors: | Nicholas T. Salzameda Joseph T. Barbieri Kim D. Janda |
| Affiliation: | 1. Departments of Chemistry, Immunology, and Microbial Science and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, CA 92037, USA;2. Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, WI 53226, USA;3. Worm Institute for Research and Medicine (WIRM), The Scripps Research Institute, La Jolla, CA 92037, USA |
| Abstract: | A FRET peptide substrate was synthesized and evaluated for enzymatic cleavage by the BoNT/B light chain protease. The FRET substrate was found to be useful in both a high throughput assay to uncover initial ‘hits’ and a low throughput HPLC assay to determine kinetic parameters and modes of inhibition. |
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