Bioaugmentation of carbofuran residues in soil using Burkholderia cepacia PCL3 adsorbed on agricultural residues |
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| Authors: | Pensri Plangklang Alissara Reungsang |
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| Institution: | 1. International Postgraduate Programs in Environmental Management, Graduate School, Chulalongkorn University, Bangkok 10330, Thailand;2. National Center of Excellence for Environmental and Hazardous Waste Management (NCE-EHWM), Chulalongkorn University, Bangkok 10330, Thailand;3. Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand;4. Research Centre for Environmental and Hazardous Substance Management, Khon Kaen University, Khon Kaen 40002, Thailand;1. Department of Chemical and Environmental Engineering, University of California, Riverside, CA 92521, USA;2. Department of Civil and Environmental Engineering, Duke University, Box 90287, Durham, NC 27708, USA;1. Chemical and Environmental Systems Modeling Research Group, National Institute of Fundamental Studies, Kandy, Sri Lanka;2. Korea Biochar Research Center & Department of Biological Environment, Kangwon National University, Chuncheon 200-701, Republic of Korea;3. School of Environmental Sciences, Jawaharlal Nehru University, New Delhi, India;1. Institute of Microbiology, Universität Innsbruck, 6020 Innsbruck, Austria;2. Dipartimento di Scienze delle Produzioni Agroalimentari e dell’Ambiente (DiSPAA), Università degli Studi di Firenze, 50144 Firenze, Italy |
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| Abstract: | Burkholderia cepacia PCL3 (GenBank accession number of EF990634) is a carbofuran degrader isolated from phytoremediated rhizosphere soil in our laboratory. Free and the immobilized PCL3 on corncob and sugarcane bagasse were investigated for their abilities to degrade carbofuran in Basal Salt Medium (BSM) and soil microcosm. The reusability and survival of immobilized PCL3 in comparison to free cells were also examined. Short half-lives (t1/2) of carbofuran of 3–4 d in BSM were obtained using the isolate PCL3 in both free and immobilized cell forms. Immobilized cells could survive (106–107 cfu ml?1) through 30 d of incubation, while the number of free cells decreased continuously after 10 d. Immobilized B. cepacia PCL3 could be reused twice without loss in their abilities to degrade carbofuran in BSM, which suggested an advantage of using immobilized cell over free cell. Free and immobilized cells were augmented into soil and showed an effective capability to remediate carbofuran residues, both of which indicated by 5-folds decrease in carbofuran half-lives in augmented soil. Immobilization of PCL3 on corncob and sugarcane bagasse provided the possibilities of reusing the cells as well as improving the cell survival without decreasing carbofuran degradation activity. |
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