Study of beta-amyloid peptide (Abeta40) insertion into phospholipid membranes using monolayer technique |
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Authors: | Ji S-R Wu Y Sui S-F |
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Institution: | (1) Department of Biological Sciences and Biotechnology, State-Key Laboratory of Biomembranes, Tsinghua University, Beijing, 100084, People's Republic of China |
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Abstract: | -Amyloid peptide (A ), a normal constituent of neuronal and non-neuronal cells, has been proved to be the major component of extracellular plaque of Alzheimer's disease (AD). The interaction of A with lipid membranes may be essential for its neurotoxicity. Our previous study revealed that membrane insertion may provide a possible pathway by which A prevents itself from aggregation and fibril formation. In the present work we studied the membrane insertion of A and the factors that affect its insertion ability using a monolayer approach. The results show that A is surface active and can insert into lipid monolayers. When a high level of cholesterol is present, A 40 can insert into the phospholipid mixtures simulating physiological membrane composition. Acidic pH enhances A insertion, while the effect of ionic strength is rather complex. A insertion ability may be ultimately relative to cholesterol-rich domains in the monolayers, which indicates strong interaction between A and cholesterol. |
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Keywords: | -amyloid peptide" target="_blank">gif" alt="beta" align="MIDDLE" BORDER="0">-amyloid peptide monolayer membrane insertion cholesterol |
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