Specific interaction of initiation factor IF2 of E. coli with formylmethionyl-tRNA f Met. |
| |
Authors: | Hans Uffe Petersen Thomas Røll Marianne Grunberg-Manago Brian F.C. Clark |
| |
Affiliation: | 1. Institut de Biologie Physico-Chimique, 13, rue Pierre et Marie Curie, 75005 Paris, France;2. Department of Biostructural Chemistry, The University of Aarhus, 8000 Aarhus C, Denmark |
| |
Abstract: | The interaction of E.coli initiation factor IF2 with formylmethionyl-tRNAfMet has been studied by measuring the inhibition by IF2 of the spontaneous deaminoacylation of the charged tRNA. We find that IF2 protects fMet-tRNAfMet against spontaneous deacylation. The formylation is an absolute requirement for this protection and no effect of GTP was found. The association constant for IF2 binding to fMet-tRNAfMet at 37°C and physiological ionic conditions was estimated at about 106 M?1. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|