Specific interaction of initiation factor IF2 of E. coli with formylmethionyl-tRNA f Met. |
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| Authors: | Hans Uffe Petersen Thomas Røll Marianne Grunberg-Manago Brian FC Clark |
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| Institution: | 1. Institut de Biologie Physico-Chimique, 13, rue Pierre et Marie Curie, 75005 Paris, France;2. Department of Biostructural Chemistry, The University of Aarhus, 8000 Aarhus C, Denmark |
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| Abstract: | The interaction of E.coli initiation factor IF2 with formylmethionyl-tRNAfMet has been studied by measuring the inhibition by IF2 of the spontaneous deaminoacylation of the charged tRNA. We find that IF2 protects fMet-tRNAfMet against spontaneous deacylation. The formylation is an absolute requirement for this protection and no effect of GTP was found. The association constant for IF2 binding to fMet-tRNAfMet at 37°C and physiological ionic conditions was estimated at about 106 M?1. |
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