Development of a sequential injection analysis system for monitoring of trehalose concentrations |
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Authors: | Jong II Rhee |
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Institution: | (1) School of Applied Chemical Engineering, Research Center for Biophotonics and Center for Functional Nano Fine Chemicals, Chonnam National University, 500-757 Gwangju, Korea |
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Abstract: | A sequential injection analysis (SIA) system was developed to on-line monitor the concentrations of trehalose in biological
processes. The system is based on the enzymatic reaction of trehalose to glucose in an immobilized trehalase reactor and the
subsequent reaction of glucose in the presence of glucose oxidase. The peroxide produced is detected using horseradish peroxidase
with a redox indicator (ABTS, 2,2′-azino-di(3-ethylbezthiazoli-sulfonic acid-6)) and a spectrophotometer set at 435 nm. The
SIA was fully automated using software written in the LabVIEWTM development environment. A number of system variablese.g. flow rate of the carrier buffer solution and volume ratio of sample to reagents, were evaluated to increase the sensitivity
and performance of the SIA system. The performance of the trehalose-SIA system was linear under partially optimized operating
conditions in the concentration range of 0.3 to 2.0 g/L (R2=0.999) with a sample frequency of 6 1/h. The SIA system was successfully employed to on-line monitor the concentrations of
trehalose in a continuously stirred tank reactor. The on-line monitored data were in good agreement with the off-line data
measured by a HPLC with a refractive index detector (n=14, R2=0.9865). |
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Keywords: | biological process trehalose monitoring sequential injection analysis |
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